* = The recommended starting sample volume in the Mouse HMW & Total Adiponectin ELISA is between 5 and 10 µl. See package insert for details (Section 7: Sample Volume Adjustment).
Adiponectin circulates as multiple isoforms1 and is widely recognized as a key protein involved in insulin regulation and metabolic syndrome. Of the different multimers, the high-molecular-weight (HMW) form of adiponectin activates adenosine monophosphate activated protein kinase phosphorylation most effectively1, and HMW levels, or the ratio of HMW to total adiponectin, are more meaningful than the total adiponectin level for predicting insulin resistance and the development of metabolic syndrome2.
Recently, an ELISA system was developed for the selective quantification of human adiponectin oligomers using protease pretreatment3, and the Mouse HMW & Total Adiponectin ELISA reflects a similar system for simultaneous detection of mouse HMW and total adiponectin. 4
1) Hada Y, et al. Biochem Biophys Res Commun 2007; 356: 487-93.
2) Hara K, et al. Diabetes Care 2006; 29: 1357-62
3) Ebinuma H, et al. Clin Chim Acta 2006; 372: 47-53
4) Ebinuma H, et al. Clin Chim Acta 2009; 401: 181-3
For more information on the Mouse HMW & Total Adiponectin ELISA, call 800-592-5726.